Anti -Cleaved- Caspase-3 Rabbit pAb

價格：在線咨詢

貨號：

KT012184

產(chǎn)品規(guī)格：

100ul

Applications：

WB/IHC/IF

Species：

H/M/R,M,M

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	Cleaved- Caspase-3 rabbit polyclonal
Protein full name	Caspase-3
Synonyms	CASP3, CPP32, CPP32B, SCA-1, caspase 3
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse caspase3
Isotype	IgG
Predicted MW.	17/35KDa
Observed MW.	17/35KDa
Purity	Affinity purification
Subcellular location	Cytoplasm
Uniprot ID	P70677,P42574,P55213

Applications

applications	species	dilution	Positive tissue
WB	Human, Mouse, Rat	1: 1000	Hela, HepG2, SW480
IHC	Mouse	1: 200-1: 1000	tumor
IF	Mouse	1: 200-1: 1000	tumor

Background

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.

Images

Western blot analysis of Cleaved-Caspase3 (GB11009) at dilution of 1:1000.

Lane 1:Hela cell lysate

Lane 2:HepG2 cell lysate

Lane 3:SW480 cell lysate

Lane 4:B16 cell lysate

Lane 5:C6 cell lysate

Immunohistochemistry analysis of paraffin-embedded mouse tumor using Cleaved-Caspase3 (GB11009) at dilution of 1:1000

Immunofluorescence analysis of paraffin-embedded mouse tumor using Cleaved-Caspase3 (GB11009) at dilution of 1:500

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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